Journal: Scientific Reports

Article Title: Porcine sperm bind to an oviduct glycan coupled to glass surfaces as a model of sperm interaction with the oviduct

doi: 10.1038/s41598-025-88986-2

Figure Lengend Snippet: Schematic representation of the two in vitro systems developed to model the sperm-oviduct interaction. ( a ) 3-D functionalized streptavidin coverslip: the coverslips are placed on a petri dish, medium droplets containing sulfated Le X (suLe X ) are placed on the streptavidin regions of the coverslip that are surrounded by hydrophobic surface, and the whole system is covered with mineral oil. ( b ) Polyethyleneglycol (PEG)-biotin slides: the slides are coated with streptavidin, a hybridization chamber is placed on the slide, and the chambers are filled with medium containing suLe X . ( c ) The streptavidin coating in both glass surfaces allows biotinylated suLe X to be tightly immobilized to the surface. Once the sperm are added and incubated in the system, membrane receptors on the head recognize and attach to suLe X bound to the glass.

Article Snippet: Two functionalized glass surfaces were used to immobilize oviduct glycans and model the oviduct reservoir (Fig. ): 3D-functionalized streptavidin coverslips (PolyAn Scientific, Berlin, Germany) and functionalized PEG-biotin slides (Microsurfaces Inc., Englewood, NJ).

Techniques: In Vitro, Hybridization, Incubation, Membrane

Journal: Scientific Reports

Article Title: Porcine sperm bind to an oviduct glycan coupled to glass surfaces as a model of sperm interaction with the oviduct

doi: 10.1038/s41598-025-88986-2

Figure Lengend Snippet: Schematic representation of IVF using sperm bound to glycans pre-fertilization (glycan-IVF). Functionalized streptavidin-coverslips were coated with suLe X in droplets of medium. Sperm were added to the droplets and incubated to allow them to bind suLe X . The medium in the droplet was replaced to remove free sperm. Mature cumulus-oocyte complexes (COCs) were added to the droplet and co-cultured with bound sperm. COCs induce the release of some of the sperm enabling them to fertilize the oocytes.

Article Snippet: Two functionalized glass surfaces were used to immobilize oviduct glycans and model the oviduct reservoir (Fig. ): 3D-functionalized streptavidin coverslips (PolyAn Scientific, Berlin, Germany) and functionalized PEG-biotin slides (Microsurfaces Inc., Englewood, NJ).

Techniques: Incubation, Cell Culture

Journal: Journal of visualized experiments : JoVE

Article Title: Preparation of Meiotic Chromosome Spreads from Zebrafish Spermatocytes

doi: 10.3791/60671

Figure Lengend Snippet: Table of Materials

Article Snippet: 24 x 60 mm glass coverslips , VWR International , 16004–312 , .

Techniques: Generated, Modification, Hybridization, Microscopy, Electron Microscopy